To the procedure of confirmation of the laboratory’s competence in performing mutagenicity assessment using the Ames test

Introduction. The test for the induction of reverse gene mutations (Ames test, OECD* guideline No. 471) is one most popular methods assessing mutagenicity due to its ease execution and ability detect up 70-80% substances with carcinogenic activity. experimental protocol requires a minimum amount substance standard microbiological laboratory equipment. To obtain primary data, several days from start experiment are required. Despite existence publications devoted detailed description Ames protocol, there gap affecting number aspects procedure confirming competence testing center using this method in practice. Materials methods. When preparing article, we used literature data published domestic foreign over past 20 years concerning approaches implementation test. search was carried out Scopus, Medline, Google Scholar, RSCI databases. Results. In FBES “Federal Scientific Center Hygiene named after F.F. Erisman” Federal Service Surveillance on Consumer Rights Protection Human Wellbeing bacterial mutation has found application safety technical products pesticides, their mixtures preparative forms, as well examination equivalence. Testing basis accredited compliance state GOST ISO / IEC 17025-2019 ”General requirements calibration laboratories.“ based new our own practical experience, necessary conditions considered demonstrating practice, reliable results take actions manage risks activities. main attention paid ensuring such parameters quality indicator cultures, metabolic activation system, control background spontaneous mutation, etc. Conclusion. discussed issues can be useful specialists research laboratories planning introduce into